Food and Feed Testing for Vitamins
Many foods are fortified with vitamins by spraying or mixing premixes into the food products. The foundation of analysis is to check if the declared vitamin content adheres to the local standards and if the marketing claims are justified. Vitamins are known to degrade with time due to oxidation and in fact the labelling contents must be equal or exceed that which is stated. In Singapore, the food regulations states that no label shall contain any nutrition claim unless it also includes a nutrition panel in the form specified in the twelfth schedule, which declare the amount of the nutrients stated. In fact, many food in Singapore must have a prerequisite amounts of nutrients, e.g. margarine shall contain in 1 kg, vitamin A of an amount of not less than 8.5mg of retinol activity. Therefore fast and reliable analytical tests is important.
There are a total of 13 vitamins of which vitamin A, D, E and K are fat soluble and the others, vitamin B and C are water soluble. Bioscience Diagnostics Singapore provides 2 tests for easy analysis of vitamins
VitaFast®
The VitaFast ® are microbiological tests in an easy to use microtiter plate format. The microorganism lyophilized on the wells grow in dependence on the vitamin concentration. This growth can be measured with turbidity using a microtiter plate reader. This is possible for all water soluble vitamins - Folic acid, Pantothenic acid, Vitamin C (Ascorbic Acid), Vitamin B1 (Thiamin), Vitamin B2 (Riboflavin), Vitamin B3 (Niacin), Vitamin B6 (Pyridoxine), Vitamin B7 (Biotin), Vitamin B12 (Cyanocobalamin), Inositol. For testing solid samples, the vitamin has to be extracted and diluted before pipetting into the wells. Liquid samples can be added directly. Incubation is usually 48hrs at 37°C. This is a quantitative test.
RIDASCREEN® Vitamin Test
The RIDASCREEN® is also a microtiter plate test for vitamin B12 and folic acid. RIDASCREEN® allows a fast and precise quantitative analysis of single added vitamin by means of an antibody antigen complex. The test require less than 1 hour. Milk and juices can be added directly onto the plates without sample preparation. This test has been validated for use in milk, milk powder, food for special medical purpose, grain and cereals, fortified flour, vitamin powder (mixture or tablets) and vitamin juice. RIDASCREEN® usually has less cross reactivity for a large number of folic acid derivatives.
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